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KMID : 0545120160260112006
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Journal of Microbiology and Biotechnology
2016 Volume.26 No. 11 p.2006 ~ p.2011
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The Anti-Rotaviral and Anti-Inflammatory Effects of Hyrtios and Haliclona Species
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Koh Su-Im
Shin Hea-Soon
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Abstract
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The marine sponges Hyrtios and Haliclona species, both of which are known to produce secondary bioactive metabolites, were used to extract 1304KO-327 and 1304KO-328. Such secondary metabolites are potentially antibacterial, antiviral, anti-inflammatory, antitumoral, antifungal, and antiplasmodial. In the present study, the effects of 1304KO-327 and 1304KO- 328 were studied for their clinical and pathological importance. The cytotoxicity of 1304KO- 327 and 1304KO-328 was assessed via MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on HT-29, Caco-2, and Raw 264.7 cells. Rotavirus-infected Caco-2 cells were used to prove the antiviral effects of the marine sponge extracts. The test results cogently proved that the virus-inhibiting effects of the sponge extracts improved with extract concentration. Anti-inflammatory effects of the marine sponge extracts were tested on Lipopolysaccharide-treated Raw 264.7 cells. Nitric oxide and cytokine were produced by treatment of the cells with LPS and the inhibiting effects of the sponge extracts on IL-1β formation were investigated. This study found that the NO production was decreased dose dependently, and IL-1β formation was significantly reduced by the marine sponge extracts.
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KEYWORD
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marine sponge, anti-rotaviral, anti-inflammation, Hyrtios species, Haliclona species
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